Did you use Buffered Peptone Water or phosphate buffer saline?

12 July 2023 1 809 Report

Do any of you find differences between plate counts (LABacteria) with BPW (0.1 % peptone) and PBS?

The problem with buffered peptone water is that you are likely to get some cell growth over time. So if you are doing your serial dilutions and plating quickly then it may not matter, but if the cells are going to be resuspended for some period of time then there might be time for the bacteria to grow.

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