I have not used this kit but we use a Lithium Chloride precipitation prior to RNAseq which works very well for us.
Did you lose some RNA during this precipitation? Why works very well? what did you consider after this treatment? The 260/280 or 260/230 ratio, the yield, the electrophoresis run?
Yes I have used it and yield is pretty good. Just you should be careful during handling of ampure beads. Do not over dry them. Be careful removing ethanol, because it will effect all the other applications.
The Lithium Chloride precipitation usually increases the 260/230 for us. Normally our samples have a good 260/280 already if you have an optimized extraction method. The other reason I like this precipitation is that you don't lose very much RNA whereas my experience of clean-up columns (we have used RNeasy) is that you lose a lot of your RNA on the column.
Yes I have it work very well. Before you clean your RNA I suggest you check the integrity of the RNA. Degraded RNA would need some adjustments otherwise it might be washed away most of it. The other part is to make sure the beads are well mixed before pipetting.
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