Andrew Taylor

2 Questions 24 Answers 0 Followers

Questions related from Andrew Taylor

How do you determine the copy number of a gene using qPCR?

I have an assembled genome which suggests that my gene of interest has 2 identical copies. I did some qPCR by setting up a genomic DNA dilution series and amplifying my gene of interest and a...

07 July 2016 4,358 2 View

Does anyone have a good protocol for extracting DNA from large amounts of field soil?

The DNA needs to be pure, clean, free of inhibitors and suitable for quantitative PCR. The current method we use is limited to 0.25g of soil and I would like to use at least 10g, more if possible.

07 July 2014 8,197 6 View