How to make an apparatus for testing membrane permeability and selectivity?

Good afternoon everyone! I just can't find drawings or a description of the creation of an apparatus for determining the permeability and selectivity of membranes in relation to gases and gas...

14 January 2021 9,205 4 View

The loss of hyperbolicity in PDE?

Question about the loss of hyperbolicity in nonlinear PDE: when complex eigenvalues appear, what is the effect on flow? I understand that we do not have general results on existence in this case,...

16 December 2020 2,571 3 View

Fixation of whole rats organs in buffered formalin?

Dear colleagues, I would like to ask you if anyone has experience with the fixation of whole rat organs (heart, lungs, liver, kidneys, brain, testes) in 10% buffered formalin. Thank you, Roman

28 October 2020 3,175 3 View

Is there some sort of classification of simple algebras with color involution?

If $A$ is a G-graded algebra then one can define on it a color involution, i.e. a bijective linear map preserving the grading such that the image of a product of two homogeneous elements is...

24 September 2020 5,416 3 View

Which is the best methodology of research in literary work analysis and comparative literature studies? studies?

I'm developing a project on migration and literature, migration in literary works.... I was thinking about discourse analysis...May anyone suggest some methodologies used in these fields of...

13 August 2020 9,742 25 View

How can you do biotynilation of antibodies in presence of gentamicin?

For some time we have used biotinylated antibodies for ELISA. Usually I use biotin N-hydroxysuccinimide ester to link biotin to antibodies (and I have used different carbodiimides for other...

28 July 2020 8,806 3 View

If you had to reference a publication which proves or at least rigorously argues that AI control problem is (un)solvable, what would you cite?

Links to such articles with some explanations would be great!

27 July 2020 7,035 6 View

What is the estimated projected price of electricity (or hydrogen) for bio-solar cells?

Hello, everyone! There are many reports about bio-based solar cells and their prospects in the scientific literature. All authors write that these devices will be low-cost. Does anyone know any...

13 July 2020 655 3 View

Is there a method to validate and reliability test high school science lesson plans for a high school science course?

Hello, I am working on my dissertation and am in the data gathering phase. Is there a recommended approach to validating and reliability testing science lesson plans within a high school. I am...

08 March 2020 585 5 View

Can you help me?

I am looking for information as to whether some insects (or spiders?) similar in pattern of colouration (red anteriorly, blackish posteriorly, white transverse bands) to some Cleridae (Clerus,...

09 February 2020 4,416 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View