Roman Martino

2 Questions 2 Answers 0 Followers

Questions related from Roman Martino

Did anyone worked with the plasmid pIB/V5-His-DEST Vector insect cells?

I am working with baculoviruses and I want to use pIB/V5-His-DEST Vector to obtain stable insect cells to produce my protein. But, I can not purchase it. Does any one can help me?

04 February 2016 1,426 2 View

Which lysis buffer should I use to purify proteins from insect cells? DNA Mucus?

I'm using a phosphate buffer (pH8) as recommended by the protocol of Quiagen (50 mM NaH2PO4 + 300 mM NaCl + 5-10mMImidazol) to get protein purification with his tag from an insect cell (using a...

09 May 2014 3,448 1 View