Which lysis buffer should I use to purify proteins from insect cells? DNA Mucus?

I'm using a phosphate buffer (pH8) as recommended by the protocol of Quiagen (50 mM NaH2PO4 + 300 mM NaCl + 5-10mMImidazol) to get protein purification with his tag from an insect cell (using a...

09 May 2014 3,439 1 View