I tried to transform DH5-alpha chemically competent cells by pCMV-dest BacMam vector from ThermoScientific (10 Kb) for storage (without insert or entry vector) on LB Amp plates. It gave no colonies. I used 500 ng/ul vector on 100 ul competent cells and spread it on 2 plates (50 ul and 400 ul).
pCMV-DEST Vector allows you to construct an engineered viral genome using Gateway® technology, restriction enzyme cloning or seamless assembly. The vector uses GatewayR technology to create an expression clone by recombining an entry clone containing your gene of interest with the BacMam pCMV destination vector (BacMam pCMV-DEST).
Important point is that in this vector ccdB gene located between the two attR sites for negative selection. So you cannot use DH5-alpha as a competent cell for the transformation of empty vector (because the presence of ccdB which is lethal gene). Instead, you must use another competent cell like DB3.1 (a HB101 derivative containing the gyrA462 allele which renders the strain resistant to the toxic effects of the ccdB gene) that is suitable for the propagation of plasmids containing the ccdB gene.
Good Luck
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