Aya Aladdin

3 Questions 2 Answers 0 Followers

Questions related from Aya Aladdin

How can I interpret RNAstructure results (RNA secondary structures prediction)?

I used a 330 bp long RNA sequence to predict its secondary structures using (RNAstructure online tool) and got 20 different structures of energy values between 105 and 107 Kcal/mol. Now I don't...

14 June 2020 6,571 0 View

How do I optimize unlabeled EMSA assay?

I am working on electro-mobility shift assay using unlabeled DNA that is a purified PCR product of a promoter sequence, and use nuclear extract for the binding reaction. For the nuclear extraction...

19 July 2018 4,966 4 View

Did anyone try transforming pCMV-dest Vector BacMam in DH5-alpha for storage?

I tried to transform DH5-alpha chemically competent cells by pCMV-dest BacMam vector from ThermoScientific (10 Kb) for storage (without insert or entry vector) on LB Amp plates. It gave no...

29 May 2017 2,566 1 View