I am looking to try BD 550319 (IL-6) BD 558535 (TNF alpha) on frozen sections any suggestions
Immunohistochemistry for qualitative only ie expressed or not and the intensity of expression.
If you want quantitative level then R PCR or Western Blot
Hello
I think yes . that an antibody designed for ELISA will work for IHC because the product used to immunized is often a peptide, and the antibody recognizes the denatured protein .
You may see technical Data Sheet or contact the technical services of the antibody companies to discuss whether those antibodies are also suitable for IHC.
I hope this may help , Good Luck
Personally, I would try polyclonals for IHC since they would recognize multiple epitopes.
Hi all, Thanks for your suggestions, Hi Stefansson do you mean all ELISA antibodies are monoclonal and All Immuno Antibodies are poly clonal....Please elaborate
Hi, in ELISA the coated antibody is usually a polyclonal since it is more likely to capture most of the antigen. The detection Ab is often a labeled MoAb to provide specificity in the detection.
This not an absolute rule because it all depends on the antigen, available antibodies and their specificities. I have seen ELISAs using 2 MoAbs or 2 polys.
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