It seems Random primer step in the 1st strand preparation is missing. Is it added to some mix? The rest closely follow previous 1st strand NEB Ultra II kit protocol NEBnext Ultra II RNA library prep kit for Illumina, E7770S/L.
Computational topology of solitons The well-established research area of algebraic topology currently goes interdisciplinary with computer science in many directions. The Topological Data Analysis...
08 July 2024 2,816 0 View
Hello Researchers! I have a question for you. Still debating... We work with human cells. Is it OK after harvesting cells to resuspend them in Fetal Bovine Serum for further counting? Will the...
05 December 2023 5,728 1 View
Hi all. I am starting to work with S. cerevisiae Y187 and I am not sure in what media it should be cultivated. Could someone please give me some tips? Thank you.
20 July 2023 2,527 2 View
I am looking for a book, that would explain in a pretty simple language the different statistical methods and their practical application in the research, the social research in particular. The...
23 March 2023 2,041 6 View
I need my FWCI
08 February 2023 3,220 1 View
Hi everyone, I'm currently working with anhydrotetracycline solution in DMSO and I couldn't find for how long I can use the same aliquot and how should I stock it. Does anyone can help me? Thanks
27 June 2021 5,052 3 View
Collagenase primarily is used for tissue digestion and TrypLE and Trypsin for attachment cells passaging. Is anybody tried to use Collagenase to lift monolayer of attached cells? If so what is the...
04 March 2021 3,470 0 View
Are there any projects/articles on BI performance metrics and BI platforms selection criteria?
26 April 2018 365 6 View
Dear all, does anybody have an experience of carbon ink preparation which consists of hydrophobic nanoparticles dispersed into water-IPA mixture and a small amount of Nafion solution? I tried...
21 November 2016 8,375 3 View
Which method do you usually use to determine the degree of ionic liquids leakege from a polymer membrane? In term of Fuel Cell application. Thank you in advance!
13 June 2016 851 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View