Did anyone here ever did a successful Western blot for either Usp9x, USP7 or HUWE1 ?

02 June 2015 1 6K Report

I do semi-dry on PVDF, block with 4% milk and do the AB 1:1000 oN but all I get is background. Any help is appreciated!

Hi Inga,

I do semi dry transfer on PVDF but i use 5% milk for blocking.

To reduce the back ground I would suggest you to give more washes after primery incubation as well as after secondary antibody.

Try to change the primery Ab dilution.

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