Dear colleague,
Please be more specific in your question so we can help you. In general, you should check the mean average size of the particles and the cutt off value of your dialysis membrane which should not be greater than the particle size. The solvent used in the purification, dialysis time and other parameters will depend on their nanoparticles.
You can also check this paper for more info: Article Influence of different cleaning processes on the surface che...
Choose suitable dispersion medium and membrane cutt off values. Load you dispersion into the bag, immerge and hang it onto a container having the washing medium, stir the washing liquid magnetically. Choose the parameters, such as dialysis time, air or inert, light/dark environments, stirring time and speed, frequency of changing the washing liquid). you can see the following:
https://www.researchgate.net/post/full_steps_of_nanoparticle_purification_by_dialysis_technique
Many thanks.
If I want to get pure nanoparticles with 20 nm, what is the best molecular weight cut off value of required dialysis bag.
In this paper the authors produced 5 nm nanoparticles using 15 kDa MWCO dialysis membrane.
J. Am. Chem. Soc., 2006, 128 (10), pp 3190–3197.
10.1021/ja0558241
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