Is there a protein concentration range available for cell culture supernatant?
(e.g. supernatant from HeLa cells)
could you help me out?
Hello Naile Merve Guven,
No, sorry, there isn't any such protein concentration range available for cell culture supernatant.
The protein concentration will vary depending on many factors such as the cell type as different cell types will secrete varying amounts of proteins, so the reference range will differ between cell lines; the composition of media can influence protein secretion; culture conditions which include factors like supplements, temperature and pH can impact protein production and the growth phase like the exponential phase vs the stationary phase can bring about fluctuations in protein secretion.
The exact range will have to be determined experimentally for your specific cell line and conditions. You may perform multiple measurements on different samples from the same cell culture using standard protein assay like BCA or Bradford to get a reliable estimate of the protein concentration. Compare your results with the published work using similar cell lines to get a general idea of expected protein concentrations.
Best,
Hello Naile Merve Guven,
In order to estimate the protein concentration from HeLa lysates, you would need to perform a colorimetric biochemical assay such as bicinchoninic ad assay (BCA) or Bradford. Compare your sample OD values with that of the standard BSA samples in order to determine the test sample concentration. To further confirm if your protein concentration is good enough, you can refer to the product manuals or literature. Although this will only help to have an idea about a standard specific range, can vary as per alterations of your experiment conditions.
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