Namita Chutani

3 Questions 3 Answers 0 Followers

Questions related from Namita Chutani

For a detection of lower molecular weight protein using SDS gel or western, what optimum conditions should follow?

Faint or no band for low molecular weight, even for ladder post gel run no band detection in western blot Have used 10.15 and 20% polyacrylamide gels voltage100-120, 60mins or more depending on...

08 June 2023 4,017 1 View

What could be the reason of lack of desired product amplification in site directed domain mutagenesis?

I have been working to generate a protein domain deletion for some time using QuikChange II Site-Directed Mutagenesis Kit by Agilent. I am not able to get the amplification either in test or...

29 September 2021 1,265 2 View

Is there any staining procedure to detect fimbriae or pillin protein specifically?

My group have been working on a small project ,as a part of which we need to detect attachment of bacteria (DH5 alpha and BL21 to be specific) to mannose expressing cells. It will be a great help...

28 June 2019 8,146 0 View