Why my protein band in SDS-PAGE if heating the sample becomes much lighter compared to not heating the sample?

I ran a SDS-PAGE of my sample ovalbumin in histidine buffer in 2x Laemmli buffer without heating and it got nice visible bands. However, when I heat the sample at 77C in 2x laemmli buffer for 5...

15 February 2021 3,080 3 View

Why cannot get any graphene on the surface of copper foil after a CVD growth process?

Parameter: 0.3 sccm CH4 + 2 sccm H2 / 1000 C / 350 mTorr / 1-15 min Pretreatment: Electropolishing + 30 min anneal with 10 sccm H2 Tried several times but get no carbon on copper, then tried to...

01 January 2021 9,754 3 View

Methods for multivariate discrete-time time series clustering?

Can somebody suggest a popular method for clustering multivariate discrete-time time-series data ? My dataset is about water quality parameters taken in 6 stations by seasons in 5 years . Thank...

09 December 2020 6,933 12 View

How to generate a homodimer from a monomer in PyMol or other software?

I performed tertiary structure prediction of my enzyme in Modeller. However, it is predicted to be a homodimer. How can I obtain a homodimer from the monomer obtained by prediction in a reliable way?

07 April 2020 5,264 2 View

Accuracy of land-use classification and uncertainties in land-use change assessment?

I am analyzing land-use change in 3 periods. For each period, one land-use map was derived from remote sensing technique along with accuracy of classification. I want to ask if there is any...

23 February 2020 8,063 5 View

Application of Mann-Kendall on daily/monthly time series flow data with seasonality?

I want to use Mann-Kendall method to detect trends in time series data. Now I have daily flow data from 1996 until recently, the problem is I learned that Mann-Kendall method is not suitable for...

06 May 2018 6,794 4 View

Analysis of Trends of water level and flow discharge?

I'm going to analyze the trend of water level and flow discharge using time series data. Can you recommend a good paper or website which focuses on this task? I have no hydrological background so...

05 May 2018 2,074 9 View

How does BIM help to increase productivity in construction industry?

I would like to know what aspects of productivity BIM could promote in different phases of a construction project.

15 March 2015 7,416 8 View

What kind of sensor can measure peat soil pH and redox in the field?

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13 March 2014 7,456 1 View

What size of moving window to calculate diversity of landscape?

Can anybody suggest a robust way to decide the size of moving window to to assess landscape diversity? I have a raster land use map with resolution 10 x 10m. Now I want to calculate diversity...

01 January 1970 10,001 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View