Why my protein band in SDS-PAGE if heating the sample becomes much lighter compared to not heating the sample?

I ran a SDS-PAGE of my sample ovalbumin in histidine buffer in 2x Laemmli buffer without heating and it got nice visible bands. However, when I heat the sample at 77C in 2x laemmli buffer for 5...

15 February 2021 3,080 3 View

How to prepare OH- terminated Si Wafer?

Hi, Folks. I am working on the ALD project to deposit Al2O3 on the Si wafer from TMA and Water. I have struggled with the preparation of OH- terminated Si wafer. I have both Si(1 0 0) and...

08 February 2021 8,792 2 View

Why cannot get any graphene on the surface of copper foil after a CVD growth process?

Parameter: 0.3 sccm CH4 + 2 sccm H2 / 1000 C / 350 mTorr / 1-15 min Pretreatment: Electropolishing + 30 min anneal with 10 sccm H2 Tried several times but get no carbon on copper, then tried to...

01 January 2021 9,754 3 View

Real Time PCR: What is the justification for acceptable variance within 3 Ct? Is there any publication on this variance?

Hi all, I was not able to find any source with evidential justification for why the acceptable variance for internal control should be within 3 Ct and not other numbers of cycle of amplification....

17 December 2020 9,481 1 View

Methods for multivariate discrete-time time series clustering?

Can somebody suggest a popular method for clustering multivariate discrete-time time-series data ? My dataset is about water quality parameters taken in 6 stations by seasons in 5 years . Thank...

09 December 2020 6,933 12 View

Can you help complete a survey, if you know/ have used LPWAN technology in smart cities/transportation applications?

IoT/LPWAN User Survey Link: https://lnkd.in/dVdK-ea ----------------- Dear Sir/Madam, We are surveying the use of Low-Power Wide-Area Network (LPWAN) technologies (e.g., LoRa, SigFox, NB-IoT) for...

05 August 2020 9,001 3 View

How can I get the correct J-integral during crack propagation?

Hi. I'm learning GTN fracture mechanics in Abaqus/Explicit. But I can't get J-integral during crack propagation. Please, help me.

07 May 2020 6,589 3 View

I am conducting forest inventory in a tropical forest but I am not a botanist. Could anyone provide me the field guides for plant ID?

The inventory will focus on trees that has a diameter >5cm. Tropical Forest of Central America For example: Gentry's field guide to the families and genera of northern South America or any...

05 March 2020 865 3 View

Accuracy of land-use classification and uncertainties in land-use change assessment?

I am analyzing land-use change in 3 periods. For each period, one land-use map was derived from remote sensing technique along with accuracy of classification. I want to ask if there is any...

23 February 2020 8,063 5 View

How to design an exercise to enhance a psychological phenomenon such as self-compassion?

- What is the principle behind practicing/enhancing self-compassion? Or other words, how is self-compassion enhanced by practicing these exercises on Kristen Neff's website:...

04 November 2019 9,866 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How UAV or drone can be beneficial to better estimate crop yield?

As we know, crop yield estimation has been done traditionally with farmer's expert knowledge, deductive sampling in representative areas, and remote sensing with satellite imagery. Farmer's Expert...

01 March 2021 3,468 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

What are the LAB methodologies (procedures, equipment etc) for CO2 enrichment experiments to study crop responses?

I am doing a proposal for a research entitled 'GRAIN YIELD AND QUALITY RESPONSES OF WHEAT GENOTYPES UNDER CO2 ENRICHMENT AND EXTREME EVENTS' I will be using climate chamber and I would like to...

18 February 2021 6,494 4 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

Can bio-decomposer be a successful alternative to reduce residue burning?

To maintain the proper crop plan most of the times there is not enough time to make the field ready for the next crop. In this particular context , can bio-decompser be a solution? What about...

16 February 2021 7,068 24 View

How much costs direct reduction of nitrogen on farmland in terms of lower crop yield?

Dear research fellows, I wonder if someone knows how much direct reduction of nitrogen on farmland costs in terms of lower crop yield expressed as EUR/kg nitrogen (or similar)? I understand that...

15 February 2021 1,323 3 View