Determination of doxorubicin in exosome by HPLC?

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Hello everyone, have anyone an experience about ligation with one digested vector(salI) with double digestion insert(SalI XhoI) that ligate together?

My vector is 7000 bps, and my insert is 1300 bps

28 May 2024 831 4 View

How can I optimize parameters of cyclic voltammetry to observe oxidation peaks?

I am investigating the redox behavior of Diene compounds by cyclic voltammetry in anaerobic environment. My electrodes include carbon glassy electrode as working electrode, platinum as counter and...

06 August 2023 8,463 3 View

How can we explain the expression changes between a total and phosphorylated form of a protein?

I am analyzing the expression of a receptor tyrosine kinases protein and its phosphorylated form via Western blots. The phosphorylated form has decreased significantly, and the total remains...

11 May 2023 9,655 9 View

Why there is no endogenous expression of my protein?

I tried to detect the endogenous expression of my protein of interest, but I can't. However, at the same time, I can verify the overexpression of the protein with different antibodies. I tried...

02 May 2023 3,547 4 View

DSP (cross-linking reagent) for suspension cells?

Hello, Has anyone ever used DSP ( a cross-linking reagent) for suspension cells before preparing cell lysate for performing co-Immunoprecipitation?

19 February 2023 2,223 0 View

Is there a solution to stain the bacteria and quench the dye and use another dye like DAPI to stain the cell?

In my project, I am trying to show Staphylococcus aureus in endothelial cells with fluorescent dyes. But when I used calcein AM, due to the presence of esterase enzyme in the bacteria and the...

28 October 2022 2,706 1 View

How to improve the concentration of input samples during immunoprecipitation?

Hello, I am doing co-immunoprecipitation to study the interaction of my protein of interest. The protein concentration after performing the Bradford assay for input samples is deficient. Does...

20 October 2022 2,267 8 View

How can I export voxel based file or tetra grid mesh from Avizo ?

I want to provide a voxel-based file or tetra grid mesh geometry for analysis in solvers like Abaqus. always generating tetra mesh face with different errors like the following figure. Does...

02 February 2022 2,090 2 View

How can I solve this error in Abaqus?

I exported the model from 3 matics to Abaqus for analysis but was faced with this error : 6766 elements have missing property definitions. The elements have been identified in element set...

24 January 2022 3,801 4 View

Simulating Px4 controller tool?

Hi everyone, I am trying to simulate Px4 controller, however I wanna change the drone dynamics. Is there any straightforward tool I can work with? The Matlab UAV toolbox's attitude controller is...

05 January 2022 8,126 4 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,492 0 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View