Whole genome sequencing is as easy as ABC now. However, is it possible for us to fully utilize the information to sort out certain fundamental understanding of the cell, for instance, determination of the cell ploidity?
PCR might introduce bias amplicons, how can I get more information of the cause and the structure of the amplicon?
03 April 2014 5,747 2 View
It is hard to get the yeast genome sequence with PacBio due to the ribosome and some other DNA binding proteins that inhibit the polymerase, any suggestions for improvement?
31 December 2013 3,298 2 View
Certain DNA binding protein DBPs might not be removed and affect the downstream activity, any suggestions to eliminate all DBPs effectively?Any way to verify the removal?
31 December 2013 8,134 4 View
Many uncharacterized proteins were found in genome annotation. These proteins are crucial to be known as it could be a protein with noble functions. However, not many strategies have been...
11 December 2013 2,355 11 View
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07 August 2013 6,632 0 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View