.
PCR might introduce bias amplicons, how can I get more information of the cause and the structure of the amplicon?
03 April 2014 5,679 2 View
It is hard to get the yeast genome sequence with PacBio due to the ribosome and some other DNA binding proteins that inhibit the polymerase, any suggestions for improvement?
31 December 2013 3,233 2 View
Certain DNA binding protein DBPs might not be removed and affect the downstream activity, any suggestions to eliminate all DBPs effectively?Any way to verify the removal?
31 December 2013 8,057 4 View
Whole genome sequencing is as easy as ABC now. However, is it possible for us to fully utilize the information to sort out certain fundamental understanding of the cell, for instance,...
11 December 2013 4,034 0 View
Many uncharacterized proteins were found in genome annotation. These proteins are crucial to be known as it could be a protein with noble functions. However, not many strategies have been...
11 December 2013 2,282 11 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...
02 March 2021 752 2 View
During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...
01 March 2021 6,729 2 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...
27 February 2021 1,965 1 View
i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis
24 February 2021 5,687 4 View
I want to understand the basics of docking, and also know about all bioinformatics tools which are free of coast and helpfull in in-silico analysis?
22 February 2021 8,879 2 View
Hi I am running a simulation in Abaqus for a concrete beam. Its fine aggregate replaced with sea-sand. I was wondering if I could find studies that measured the ultimate strain of concrete made...
22 February 2021 6,409 2 View
We are conducting survey research on COVID-19 misinformation among students. How can we validate our survey for it to produce valid and reliable data? Also, what statistical tools can we use to...
16 February 2021 678 3 View
Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...
15 February 2021 8,982 2 View
