Joseph Koh

6 Questions 5 Answers 0 Followers

Questions related from Joseph Koh

Can anyone suggest where to find more information on bias amplicons?

PCR might introduce bias amplicons, how can I get more information of the cause and the structure of the amplicon?

04 April 2014 5,722 2 View

Removal of DNA binding protein during gDNA purification?

Certain DNA binding protein DBPs might not be removed and affect the downstream activity, any suggestions to eliminate all DBPs effectively?Any way to verify the removal?

01 January 2014 8,101 4 View

How to perform yeast genome sequencing with PacBio RSII?

It is hard to get the yeast genome sequence with PacBio due to the ribosome and some other DNA binding proteins that inhibit the polymerase, any suggestions for improvement?

01 January 2014 3,269 2 View

Do you have any strategies to determine 'uncharacterized protein' in wet lab?

Many uncharacterized proteins were found in genome annotation. These proteins are crucial to be known as it could be a protein with noble functions. However, not many strategies have been...

12 December 2013 2,329 11 View

Determination of cell ploidity using existing genome information?

Whole genome sequencing is as easy as ABC now. However, is it possible for us to fully utilize the information to sort out certain fundamental understanding of the cell, for instance,...

12 December 2013 4,083 0 View

Anyone uses Avadis-NGS for RNA-seq analysis? Care to share your experience?

08 August 2013 6,602 0 View