Can anyone suggest where to find more information on bias amplicons?

PCR might introduce bias amplicons, how can I get more information of the cause and the structure of the amplicon?

03 April 2014 5,679 2 View

Removal of DNA binding protein during gDNA purification?

Certain DNA binding protein DBPs might not be removed and affect the downstream activity, any suggestions to eliminate all DBPs effectively?Any way to verify the removal?

31 December 2013 8,057 4 View

Determination of cell ploidity using existing genome information?

Whole genome sequencing is as easy as ABC now. However, is it possible for us to fully utilize the information to sort out certain fundamental understanding of the cell, for instance,...

11 December 2013 4,034 0 View

Do you have any strategies to determine 'uncharacterized protein' in wet lab?

Many uncharacterized proteins were found in genome annotation. These proteins are crucial to be known as it could be a protein with noble functions. However, not many strategies have been...

11 December 2013 2,282 11 View

Anyone uses Avadis-NGS for RNA-seq analysis? Care to share your experience?

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07 August 2013 6,555 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

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02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View