Atopic dermatitis (AD) in children usually get better with increasing age-Why?

Sixty percent of children with AD are seen to clear of the disease by 10 years of age. It happens due to age specific changes in immunity and skin barrier function. What is/ are the immunological...

01 January 1970 6,083 4 View

Are you in favour of prescribing systemic corticosteroids in all cases of erythroderma?

Despite diverse etiologies in erythroderma , the end stage immunologic pathways are similar. As results there are similar clinical findings irrespective of causes. But in some cases systemic...

01 January 1970 7,201 4 View

Gross national happiness (GNH) index.

GNH index is used to measure the collective happiness and well being of a population. How is it calculated? Is it a substitute for gross domestic products (GDP)?

01 January 1970 2,210 2 View

Umbrella- the best way to protect your skin from sun damage.

Sun damage is a major issue in relation to skin health. I think a good quality umbrella gives better protection to skin than any other good quality sunblock. What is your opinion?

01 January 1970 4,385 2 View

Early detection of dengue.

We go for NS1 (antigen) testing to diagnose dengue. In some cases NS1 may be negative. Antibodies take a few days to develop. How dengue could be diagnosed prior to development of antibodies...

01 January 1970 8,404 9 View

Criteria of an ideal teacher.

Teaching is a noble profession. A teacher is he, who is engaged in teaching. A teacher helps students to acquire knowledge, competence and virtue (Wikipedia). In many cases we use the term ideal...

01 January 1970 6,846 16 View

RG members stats over view: A question.

Two RG member's stats over view are as follows: Member 1: 175 reads and 229 citations Member 2: 302 reads and 679 citations How the number of citations could be more than number of reads? Can a...

01 January 1970 9,433 7 View

Most effective therapeutic option for seborrhoeic dermatitis (SD).

SD runs a very chronic course like that of Diabetes or hypertension but SD is not asymptomatic. It produces many symptoms. Pruritus is one of them which is sometimes unbearable. Many treatment...

01 January 1970 7,149 3 View

Prenatal Vit-D supplementation.

Prenatal Vit-D supplementation is a common practice. Is there any positive impact of these supplementation on fetal health?

01 January 1970 8,647 4 View

First line systemic therapy for moderate to severe atopic dermatitis in children.

01 January 1970 8,245 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View