To design a specific primer for circular RNAs, are there any special conditions compared to the normal primer design? what is your suggestion?
A general consideration would be to check for any secondary structure in silico as some can have hairpins which are structures you want to avoid with primer design.
15 December 2020 7,284 2 View
I am looking for a research paper about the mathematical or computational modelling of protein oxidation (caused by reactive oxygen species).. I would really appreciate that if someone helps me...
17 November 2020 2,340 3 View
I am looking for a research in which the finite element model was trained based on experimental results.
02 October 2020 9,277 19 View
18 September 2020 5,802 5 View
This design is to check for rupture in the steel element and the behavior of the bolt does not matter.
14 August 2020 6,580 2 View
I modeled a two-span concrete box girder bridge base on FHWA design specification, and now I recognize a problem in modeling because my first period is higher than I expected. With initial...
12 July 2020 415 3 View
I am trying to run my VUMAT code with a model in Abaqus/explicit. After that the run has been completed, I observed that the stresses have been evolved in only one element as shown in the attached...
27 May 2020 5,506 3 View
I have some raw DSC test data and I need to do baseline correction. I don't know how should I do it. I would appreciate it if someone can help me with this.
16 May 2020 8,273 7 View
Hello all, Actually, I am dealing with an issue that I can't get desirable results out of my curve-fitting problem. I used lsqcurvefit in the first place. Then, I added MultiStart to my code as...
30 April 2020 4,943 2 View
I'm researching the Self - Adaptive System of hospital systems.But I've had trouble figuring out how to do organic computing for self-adaptation in hospital systems.I am looking for an article or...
14 April 2020 1,743 3 View
I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...
03 March 2021 8,066 4 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...
02 March 2021 811 3 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...
02 March 2021 8,437 2 View
Please provide Book Title and author name
02 March 2021 9,059 3 View
We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...
02 March 2021 2,300 1 View
I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...
01 March 2021 360 7 View
I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...
28 February 2021 606 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View