Which journal is suitable to publish ISI-low impact factor for protein modeling/bioinformatics/structural biology?

I want to publish manuscript related to protein modeling with low impact factor,indexed by ISI, scopus..

01 February 2015 8,040 6 View

How can I predict and evaluate this DOPE profile?

Can anyone give comments for this DOPE profile? Thank you.

31 December 2014 5,452 1 View

How to download this article? thanks.

Floral volatiles from biosynthesis to function

11 December 2014 7,418 0 View

Hi,what is the suitable modelling server for the size of amino acid sequences, ranging from 300 to 450 amino acid residues?

I am a novice user for using protein bioinformatics tools. I don't know the clear path for analyzing my amino acid sequences of interest for modelling 3-D structure. What is the initial step of...

11 December 2014 8,533 3 View

How to open Geno3D?

11 December 2014 6,839 0 View

How can one use TSP-PRED properly for submitting protein sequences?

11 December 2014 3,102 6 View

How to change to .ali file and .py file?

My current files for modeller 9.14 (Mod9.14) were all in notepad, except for protein sequences in pdb format. I dont know how to change my files to .ali and .py format. Any suggestion?

11 December 2014 9,484 2 View

Can someone advise on purified his-tag protein in elution buffer containing 500 mM imidazole (His Spin Trap kit - GE Healthcare)?

I eluted purified his-tag protein in 200 ul of elution buffer containing 500 mM imidazole. Then, I want to use it for SDS-PAGE to confirm the presence of specific his-tag protein band on gel. What...

07 August 2013 5,923 12 View

Heartburn. Is it an early warning sign of cancers?

Is it true that the use of H2 blocker, proton pump inhibitors and other antacids can lead to the progression of recurring heartburn? The effect of reducing stomach acid can also make gastrin to be...

06 July 2013 2,029 5 View

Separating gel does not solidify. How to troubleshoot?

Is it necessary to put any liquid on top of TEMED-treated separating gel mixture between spacer plates with 1.0 mm Integrated spacers in closed casting frame?If yes, what type of liquid...

06 July 2013 171 46 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View