Depc and dmpc. - FAQS.TIPS

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Which journal is suitable to publish ISI-low impact factor for protein modeling/bioinformatics/structural biology?

I want to publish manuscript related to protein modeling with low impact factor,indexed by ISI, scopus..

01 February 2015 8,166 6 View

How can I predict and evaluate this DOPE profile?

Can anyone give comments for this DOPE profile? Thank you.

31 December 2014 5,580 1 View

How to download this article? thanks.

Floral volatiles from biosynthesis to function

11 December 2014 7,538 0 View

Hi,what is the suitable modelling server for the size of amino acid sequences, ranging from 300 to 450 amino acid residues?

I am a novice user for using protein bioinformatics tools. I don't know the clear path for analyzing my amino acid sequences of interest for modelling 3-D structure. What is the initial step of...

11 December 2014 8,654 3 View

How to open Geno3D?

I cant open it after clicking its web link on google.

11 December 2014 6,963 0 View

How can one use TSP-PRED properly for submitting protein sequences?

I've got problems during filling the submission form for protein sequences. After filling the form and pressed the submit button, it stated as "can't open file". How can I solve this? thanks.

11 December 2014 3,233 6 View

How to change to .ali file and .py file?

My current files for modeller 9.14 (Mod9.14) were all in notepad, except for protein sequences in pdb format. I dont know how to change my files to .ali and .py format. Any suggestion?

11 December 2014 9,613 2 View

Can someone advise on purified his-tag protein in elution buffer containing 500 mM imidazole (His Spin Trap kit - GE Healthcare)?

I eluted purified his-tag protein in 200 ul of elution buffer containing 500 mM imidazole. Then, I want to use it for SDS-PAGE to confirm the presence of specific his-tag protein band on gel. What...

07 August 2013 6,039 12 View

Separating gel does not solidify. How to troubleshoot?

Is it necessary to put any liquid on top of TEMED-treated separating gel mixture between spacer plates with 1.0 mm Integrated spacers in closed casting frame?If yes, what type of liquid...

06 July 2013 305 46 View

Heartburn. Is it an early warning sign of cancers?

Is it true that the use of H2 blocker, proton pump inhibitors and other antacids can lead to the progression of recurring heartburn? The effect of reducing stomach acid can also make gastrin to be...

06 July 2013 2,143 5 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View