Seeking your valuable suggestions for different methods with purpose of techniques.
Biochemical tests are the tests used for the identification of bacteria species based on the differences in the biochemical activities. There are at 21 tests such as Indole, MR, VP, Citrate and so...
04 May 2018 648 0 View
Fish vaccines are administered by 3 ways, Injection, Immersion and Oral. Each of the route has some advantages and disadvantages. Norwegian salmonids are vaccinated mainly by Injection route....
04 May 2018 546 3 View
Tilapia is likely to be the most important of all aquaculture fish in 21st century, often called "Aquatic Chicken". 17α-methyl testosterone (MT) is widely used for sex reversal of of tilapia...
04 May 2018 3,660 15 View
Two fish are found to death in cultured pond. I'm looking for the cause- investigation is going on---
03 April 2018 9,598 3 View
We know pangasius (Pangasianodon hypophthalmus) is a voracious omnivore fish, I'm going to formulate a feed to carry out research by pangasius fingerling. Please suggest me how much protein %...
03 April 2018 9,614 10 View
No doubt 'Charles Darwin's Evolution Theory' had tremendous impact on modern biological sciences. To become more science-based rather than religious belief, is it really important to think and...
03 April 2018 5,385 20 View
China is a major player in global aquaculture, contributing 61.62% of total production, where as India ranked 2nd, contributes 6.62%. Together they produced nearly 70% of earth aquaculture...
03 April 2018 8,654 11 View
Fish having lack of bone marrow and lymphatic nodules, present in mammals. Which organ is the key organ for the fish for defence mechanism?
03 April 2018 5,698 0 View
Defense mechanism of vertebrates fall into two broad categories: innate and adaptive immune system, how many years ago and which evolution era they evolved?
03 April 2018 9,490 7 View
Histology is a powerful technique to see the structural changes in the cellular and tissue level. But the whole process at lease 48 hrs to be completed. Is there any short-cut so that we can...
03 April 2018 4,163 10 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View