Is there any 1/2 biochemical test for identification of Aeromonas hydrophila?

Biochemical tests are the tests used for the identification of bacteria species based on the differences in the biochemical activities. There are at 21 tests such as Indole, MR, VP, Citrate and so...

04 May 2018 334 0 View

Which route (Oral/Injection/Immersion) of vaccine will suitable for Indian Aquaculture?

Fish vaccines are administered by 3 ways, Injection, Immersion and Oral. Each of the route has some advantages and disadvantages. Norwegian salmonids are vaccinated mainly by Injection route....

04 May 2018 353 3 View

Are hormone treated fishes (Tilapia) safe for human consumption?

04 May 2018 3,466 15 View

What are the probable cause of death of two fish?

Two fish are found to death in cultured pond. I'm looking for the cause- investigation is going on---

03 April 2018 9,381 3 View

What percentage of protein in feed is needed for Pangasius fingerling culture?

We know pangasius (Pangasianodon hypophthalmus) is a voracious omnivore fish, I'm going to formulate a feed to carry out research by pangasius fingerling. Please suggest me how much protein %...

03 April 2018 9,375 10 View

Is it necessary believing in 'Darwin's Evolution Theory' while studying and working with Science?

No doubt 'Charles Darwin's Evolution Theory' had tremendous impact on modern biological sciences. To become more science-based rather than religious belief, is it really important to think and...

03 April 2018 5,012 20 View

Why fishes are not vaccinated in Chinese and Indian Aquaculture?

China is a major player in global aquaculture, contributing 61.62% of total production, where as India ranked 2nd, contributes 6.62%. Together they produced nearly 70% of earth aquaculture...

03 April 2018 8,467 11 View

What is the most important immune organ of fish?

Fish having lack of bone marrow and lymphatic nodules, present in mammals. Which organ is the key organ for the fish for defence mechanism?

03 April 2018 5,506 0 View

How many years ago innate and adaptive immune system arose in vertebrates?

Defense mechanism of vertebrates fall into two broad categories: innate and adaptive immune system, how many years ago and which evolution era they evolved?

03 April 2018 9,259 7 View

Is there any short-cut techniques for histological slide preparations?

Histology is a powerful technique to see the structural changes in the cellular and tissue level. But the whole process at lease 48 hrs to be completed. Is there any short-cut so that we can...

03 April 2018 3,977 10 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View