Dear fellows I want a help regarding finding a chromosomal position of missense variants from NCBI site?

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Leaf area of tomato ?

Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of a tomato? Can you explain with an example and what is the substitution of Ln and ln?

06 August 2024 2,508 2 View

Standard curve of H2O2?

Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...

26 July 2024 3,460 2 View

How to do FEL analysis?

In molecular dynamics simulation, to get FEL analysis, I got an error. My Python version is 3.10.7. My input files are made with a lower version of Python. But the final command to generate the...

23 July 2024 5,646 2 View

Systematic review meta-analysis paper?

Hi everybody, We are trying to write a systematic review meta-analysis paper. But I could find 19 references. I think 19 references are not enough to do a meta-analysis section and it is better to...

10 July 2024 5,490 5 View

Cut PDMS sheet with Graphtec cutter plotter CE6000-40?

Dear All, I want to cut PDMS sheet with thickness of 0.8 mm to rectangular shape with Graphtec cutter plotter CE6000-40. What condition do you offer? Do you offer any kind of special speed or force?

25 June 2024 8,111 1 View

Who can recommend where to buy Flow Cells for Illumina HiSeq 2500?

I recently acquired a used Illumina HiSeq 2500 and am in need of flow cells compatible with this machine. As I am relatively new to this aspect, I would greatly appreciate any recommendations on...

22 June 2024 5,992 1 View

IL-2 for PBMCs ex-vivo stimulation, best Concentration?

Hello everyone, I am isolating PBMCs and stimulating them using different stimulation conditions. my question is, I want to add IL-2 to the medium for better viability after stimulation, Would you...

16 June 2024 928 1 View

Ultrasonic degradation of Pectin?

Dear researchers, Greetings. We have subjected pectin solution to ultrasound to produce oligosaccharides. Initially, as the ultrasound time was increased, the molecular weight decreased....

13 June 2024 881 3 View

Why not enough quality and quantity in DNA extraction gel electrophoresis?Extraction?

Hello every body I use CTAB method for Plant DNA extraction and I need DNA for downstream steps. Unfortunately I have problem in my plant DNA extraction, quality and quantity not as expected, and...

10 June 2024 6,610 2 View

Animal feed probiotic bacteria market?

I would be pleased if you could share the report "Animal feed probiotic bacteria market for aquatic animals, BY REGION, 2022-2032" with me.

03 June 2024 255 5 View

How to use NCBI datasets ?

I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...

20 July 2024 1,339 2 View

How to download fasta files in "gene features" format from NCBI with multiple samples in R?

Hello, I was attempting to download some sequences data from NCBI. The format "gene features" could help me extract certain gene fractions using the names, but I found that it seems to be...

04 July 2024 9,875 1 View

Problems to find a region of a Virus gene?

We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...

01 July 2024 7,644 3 View

Query Regarding tRNA Annotation Order in Feature Table for NCBI Submission?

Dear Sir, I hope this message finds you well and in good health. I am writing to seek your guidance regarding an issue I encountered during the annotation of my phage sequence using DNA Master....

30 June 2024 8,650 0 View

Is there any bioinformatic tool that can download batch DNA seq and translate them into multiple aa seqs?

Hi, everyone I would like to know if there is there any bioinformatic tool that can download batch DNA seq using imported NCBI accession numbers (upto 100 sequences) and translate them into...

19 June 2024 1,917 2 View

How to design primers and run PCR in SerialCloner?

I am working on a paper for my bioinformatics class, where we are running an analysis of transcriptomics on A. thaliana col-0 about the difference in gene expression in the temperatures of 21 and...

12 June 2024 8,822 1 View

How to upload 16S and metagenomic data from fecal samples to NCBI SRA database?

Hi there, I have some 16S and metagenomic sequencing data from mice fecals and I want to upload them to NCBI Sequence Read Archive (SRA). I don't know what NCBI package is suitable for my animal...

03 June 2024 9,451 3 View

Is there any replacement for primer blast (NCBI) to see if our primers are specifically designed for our desire PCR product?

when I insert my primers in primer blast site and push the button "get primers" I just reach different errors and I don't know what the problem is!

22 April 2024 8,001 5 View

How much time does NCBI GenBank takes for public release of complete chloroplast genome sequence?

We have assembled a chloroplast genome of angiosperm and submitted the annotated assembly to NCBI GenBank. Within two-working days we got accession number for that submission. GenBank in their...

21 April 2024 9,974 0 View

What software can I use to compare two annotated genomes downloaded from NCBI database?

Which software to compare two genomes

09 April 2024 4,571 2 View