We use isofluorane anesthesia when we do intranasal administration in mice. Volume has a strong effect on where the inoculum ends up. We use two inoculation volumes, 2ul for delivery to the upper airway, and 20ul for delivery to the lower airway. Anesthesia is absolutely necessary for the higher volume. However, we use it for all i.n. procedures to minimize stress.

Further, it helps to watch the breathing of the mouse for a few breaths to time your administration during inhalation.

We use isofluorane anesthesia when we do intranasal administration in mice. Volume has a strong effect on where the inoculum ends up. We use two inoculation volumes, 2ul for delivery to the upper airway, and 20ul for delivery to the lower airway. Anesthesia is absolutely necessary for the higher volume. However, we use it for all i.n. procedures to minimize stress.

Further, it helps to watch the breathing of the mouse for a few breaths to time your administration during inhalation.

Thank you very much, Jonathan. And we rightly understand that is the inhalation with use the equipment for anesthesia?

Hi, 

 I have done also in the past intranasal admnistration in mice. You can anesthesize mice by intramuscular injection of 150µl of a solution containing 10 µg/ml Ketamine and 10 µg/ml  Xylazine. You have to inoculate your mice with a maximum volume of 25 µl intranasal... this is an extract of my Article (https://www.researchgate.net/publication/236184584_Assessment_of_efficacy_of_antifungals_against_Aspergillus_fumigatus_the_value_of_real-time_bioluminescence_imaging) paragraph of material and methods-Section "Murine infection and in vivo ....." . Best wishes

Article Assessment of Efficacy of Antifungals against Aspergillus fu...

Thank's, Célimène.

Isofluorane anesthesia and up to 10uL of volume per nostril.

I have the same doubt, because many papers describe infections or one shot treatment by intranasal route using anaesthesia, but in case of a daily treatment, I'm not sure if it's allow to apply anaesthesia everyday. Is somebody facing the same problem?

hi Caio Andreeta Figueiredo,

I am going to anesthesia mice daily (at least 5 days). However, I am also wondering like you.Did you get any answer for the above question?

Just in case anyone is having same Issue,

here's a really good method explained in video from Jove,

https://www.jove.com/video/4440/intranasal-administration-of-cns-therapeutics-to-awake-mice

Credits to the author