Tran Dat

4 Questions 8 Answers 0 Followers

Questions related from Tran Dat

Hemagglutinin inhibition (HI) assay kit recommendation?

I am going to carry out HI assay on the 2009pdm H1N1influenza virus and A549 cells. Could you plz suggest any HI kits are available? Thank you in advance. Dat

07 July 2018 1,422 0 View

What methodology to find an inhibitory mechanism on influenza A/H1N1 virus infection?

Dear everyone, I found an inhibitor (a macrolide without antibacterial activity) against influenza A/H1N1 infection by reducing viral titer and M1 gene expression. Now, I am looking for some...

09 September 2017 4,313 0 View

Is it necessary to wash cells with PBS prior to addition of culture medium?

I am going to detect the M1 gene expression from influenza-infected A549 cells and detection of viral ability using plaque assay from the culture supernatant. As protocol, A549 cells were infected...

02 February 2017 7,118 10 View

Has anyone have experiences about MTT Assay in A549 cells line?

I'd like to test a cytotoxicity effect of a drug by MTT assay. How density of A549 cells can I seed at 96 well-plate before treatment? What compound used as a positive control for them? Thank...

01 January 1970 2,476 9 View