I cannot seem to be able to dissolve Batimastat in aqueous solutions after initial reconstitution in DMSO. It always precipitates.
I have been trying to purify a protein (from Sf9 cell conditioned media) using anion exchange Chromatography followed by a Nickel column. After the Ni column, I buffer exchange the eluate to...
21 February 2022 7,121 1 View
Hello fellow researchers, I would like to know if any of you have tried both a Qubit Fluorometer and also a Nanodrop on the same samples (DNA, RNA or protein) and which you deemed as more...
13 December 2021 7,876 4 View
Hello fellow researchers, I have to purify protein from conditioned cell media supernatant. I wanted to test out how well I can utilise centricons in concentrating and "buffer exchanging" my...
22 November 2021 8,132 3 View
When performing a miniprep using the Qiagen miniprep kit on an ON culture of chemically transformed DH5alpha cells, I noticed blue spots, perhaps blue precipitate, in my miniprep samples after...
05 August 2021 9,100 0 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View
I am conducting free radical polymerization using Kraft lignin and MMA, with DMSO serving as the solvent and there is also an initiator. Following the reaction, I obtain a solution, but I am...
15 July 2024 9,827 1 View
I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...
11 July 2024 4,832 0 View
Hi All: I need to precipitate extracellular protein from supernatant. A bit confused with ammonium sulfate calculation. Should I be adding: 1. 65g of ammonium sulfate in 100 ml of...
09 July 2024 5,508 0 View
I synthesize silver nanoparticles ,and reserve it in water. Then, when I sonicate it some minutes or longer ,it couldn't be dispersed, most of them aggregate .Otherwise ,when I sonicate several...
09 July 2024 7,335 0 View
I have a very polar alcohol (soluble in MeOH, H2O, partially in heated DMSO). And a long chain fatty acid that I would like to couple. I can't find a common solvent for them. I can form the...
24 June 2024 9,319 0 View
I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...
19 June 2024 2,590 0 View
Does someone know any precipitating agent to precipitate glucose from 10 % glucose solution? or to remove glucose from solution
13 June 2024 438 0 View