Ahmed Ibrahim Akhtar

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Questions related from Ahmed Ibrahim Akhtar

Dear all, Has anyone had success in dissolving Batimastat( BB-94) in aqueous solutions? How did you manage?

I cannot seem to be able to dissolve Batimastat in aqueous solutions after initial reconstitution in DMSO. It always precipitates.

17 May 2023 2,697 0 View

Losing protein after buffer exchanging on a PD10 (GE healthcare)?

I have been trying to purify a protein (from Sf9 cell conditioned media) using anion exchange Chromatography followed by a Nickel column. After the Ni column, I buffer exchange the eluate to...

22 February 2022 7,121 1 View

What is the difference in sensitivity between a Nanodrop and a Qubit Flex Fluorometer?

Hello fellow researchers, I would like to know if any of you have tried both a Qubit Fluorometer and also a Nanodrop on the same samples (DNA, RNA or protein) and which you deemed as more...

14 December 2021 7,877 4 View

Anyone used centricons to concentrate and buffer exchange large volumes (ca. 1 litre) of protein?

Hello fellow researchers, I have to purify protein from conditioned cell media supernatant. I wanted to test out how well I can utilise centricons in concentrating and "buffer exchanging" my...

23 November 2021 8,133 3 View

Why are there dots of blue in my miniprep samples after addition of P2?

When performing a miniprep using the Qiagen miniprep kit on an ON culture of chemically transformed DH5alpha cells, I noticed blue spots, perhaps blue precipitate, in my miniprep samples after...

06 August 2021 9,101 0 View