Hello. I am searching for a DNA synthesis and cloning company that can synthesise and clone a custom fragment into a custom vector. The problem is that the fragment has 50 repeated triplets (CAG).
I know that for some reason this is a harder task for de novo synthesis. I presume that it's because it's very difficult to do quality control (usually done by sequencing, and repeated sequences are notoriously hard to sequence accurately).
Has anyone successfully synthesised or used a synthesis service to produce constructs containing repetitive sequences?
Thank you in advance.
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