19 March 2024 1 6K Report

Hi, I'm doing my first lab internship and I have a little problem, I fixed some fibroblasts with 70% cold ethanol and then I treated with gentian violet to study their morphology. Now I need to count the cells, but there are many of them. Is it possible to solubilize the violet and treat with DAPI? so I could count them faster in ImageJ

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