We evaluated the melanin synthesis and tyrosinase activity for different ingredients in B16 cells, resulting clearly whitening agents. However, when we performed the same assays in human melanocytes, none activity was found or even enhanced pigmentation. One colleague told me that B16 produces many false negatives but I don't find information that support this theory. Someone know about that?
In general you can deliberately set the false negative rate (sensitivity) or false positive rate (specificity), but not both. A common approach is to set the false negative rate to 0.05 (95% sensitivity). Doing this, however, requires acceptance of whatever level of specificity occurs at this setting. If false positives are more detrimental than false negatives, then set the specificity to a tolerable level and accept whatever level of false negatives (missed positives) occurs as a result.
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