What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

Is there an alternative to PGLS that considers phylogenetic signal when calculating the estimated value?

I have been working on a study trying to estimate body mass in mammals using a skeletal proxy in R. My dataset has high phylogenetic signal (Pagel's Lambda = 0.9) and I am interested in removing...

26 January 2021 6,997 3 View

Are phylogenetic independent contrasts still used in biology?

I have a regression equation in which I am curious if the amount of scatter around the regression line is driven by phylogeny. I know there is a lot of phylogenetic signal because Pascal's lambda...

22 December 2020 937 1 View

What is the cause of abnormal breathing in anaesthetized rats?

Is anyone able to tell me why my anaesthetized rats often do a 'double breath', or diaphragmatic breath, like a hiccup (see video). This often begins after 1-2 hours under anesthesia, and can...

06 December 2020 2,514 5 View

Problems detecting miR-155 with Advanced Taqman miR kit?

18 October 2020 9,880 3 View

Control miRs for RNA extracted from FFPE DLBCL samples?

04 August 2020 1,195 1 View

Can the magnetic permeability and electric permittivity of the vacuum be modeled as the quantum vacuum magnetization and polarization, respectively?

If the magnetic field permeability and electric field permittivity of the quantum vacuum at a point in spacetime may be understood as the effective vacuum magnetization and vacuum polarization,...

04 June 2020 7,655 2 View

What sample size do I need if my total population is 195 (countries in the world)?

Hi everyone, (Please excuse the ambiguity within the question - protecting my idea) I recently discovered some indexes which I and colleagues would like to see if scores on these indexes across...

02 June 2020 3,960 11 View

Has anyone had issues with automated plagiarism detectors and pre-prints?

I've heard of automated plagiarism systems provided by many journals nowadays picking up on similar content in published abstracts from conferences etc. and since these automated systems are a...

21 March 2020 4,304 4 View

When do I remove participants instead of impute data?

I have a data set of around 1630. Some variables are missing in 20% of the sample (the later questions - survey fatigue/drop out). (I'm considering removing 250 participants who didn't answer...

17 March 2020 7,667 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View