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Questions related from Russell B Foxall
I have a recipe for media to be used to culture human Breast Myoepithelial cells. It includes Insulin at 5ug/ml, but doesn't state the species of origin of the insulin. Would it matter whether I...
17 May 2021 4,439 0 View
We have been assessing miR-155 in RNA recovered from FFPE DLBCL samples. We found that using the TaqMan® standard Taqman miR kit, which require a separate RT reaction for every miR to be studied,...
19 October 2020 10,177 3 View
I have been running a small test experiment, extracting RNA from FFPE DLBCL blocks, and analysing miRs in the samples. So far I have been using the TaqMan™ MicroRNA Assay, which require a...
05 August 2020 1,463 1 View
Prior to lockdown, I extracted RNA from FFPE material: 2 10 micrometer thick curls per sample using the RNeasy FFPE kit. At the time I was able to start processing the samples within 30 minutes...
28 July 2020 6,520 2 View
Hi, I am going to use the GentleMACS system, with heating units, to dissociate a Human Lymph Node sample. We currently use the system to dissociate human kidney, bladder and pancreas samples,...
09 March 2020 4,047 0 View
Does anyone have any experience of staining Breast cancer sections with the following antibodies from DAKO (Agilent): Rabbit anti-human Eostrogen receptor α Clone: EP1, Cat#: M3643. Mouse...
25 June 2018 3,357 1 View
We have been using the anti-Human CD16 clone H27 antibody for immunohistochemistry of sections from formalin-fixed paraffin blocks. We need to re-order it but Biorad apparently no longer make...
15 February 2017 4,871 2 View
I currently use Liberase TL to digest collagen droplets, and also isolate TILs from tissues. This product is currently not available from Sigma (the sole supplier), Liberase TM is too harsh, and...
05 January 2017 1,293 7 View
