CTAB dna extraction method for whiteflies?

More Kamesh Krishnamoorthy Kalyankumar's questions See All

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What dilutions of serum sample should I use in ELISA?

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Sequence identity matrix?

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10 January 2020 1,339 0 View

Between Julia and MATLAB, which would be the most efficient for a discontinuous Galerkin FEM code?

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Is the Zero Shot Learning a good current research area or not?

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Any simple protocol for whitefly DNA extraction?

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RNA Extraction Using Hot Borate Method No Longer Working?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Does crude extraction using NaOH and Tris work well with Fungi?

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Low-yield gel extraction problem?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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Daniel R Sosa-Gómez

Dear Kamesh , my suggestion is: use a method based on chelex. You can use with just one specimen.

Abhijeet Singh

Hi Kamlesh,

I would suggest you to stick to basic method if you are not sure what method you are going to use. The classical phenol:chloroform:isoamy alcohol method could work fine and I could expect a fair amount of good quality DNA sufficient enough for PCRs