Cryopreserving cell sheets

More Macarena Lucia Fernandez Carro's questions See All

¿Where to buy the Aspergillus oryzae NSAR1 strain?

Greetings: I am looking to buy/obtain the auxotrophic strain Aspergillus oryzae NSAR1, but I just have found one culture collection from Japan but haven't received any answer. Could you provide...

11 July 2024 6,847 0 View

What is the optim temperature for protein denaturation in supernatants??

Hello! I´m developing a sandwich ELISA. My assay recognizes protein denaturalized very well, but there isn´t recognition when it is in its native form. So maybe if I heat the sample I could detect...

18 April 2024 5,671 3 View

Problems isolating human neutrophils using Percoll - Erythrocytes not pelleting?

Hello all, I present here a problem to see if someone has encountered it and can provide some clarity. We are trying to isolate neutrophils from patient blood in tubes EDTA. We are following the...

10 March 2024 4,156 3 View

Do my iPSCs have a normal morphology?

Hello everyone, I am starting to grow the SCTi003-A iPSC line for the first time and I am facing some drawbacks. Due to some problems with the first pass, I waited a bit longer than the 7-8 days...

22 February 2024 8,052 2 View

Transcriptomic data sets with Staurosporine?

Hi, Does anyone know about a public transcriptomic dataset (RNAseq, microarray) of Arabidopsis (or other plants) treated with Staurosporine? I checked in GEO or SRA, but I couldn't find any....

11 February 2024 8,621 0 View

Potassium Permanganate titration- more hydrogen peroxide detected than what is actually present in solution?

I have been performing titrations of a known concentration of hydrogen peroxide using 0.01N potassium permanganate and consistently requiring more potassium permanganate than what is estimated to...

07 February 2024 446 3 View

How can I resolve this convergence issue in the Abaqus CAE software?

I am conducting a sequential heat transfer-stress analysis for a composite wall consisting of steel plates and a concrete core, similar to concrete-filled tubes or CFTs. My analysis involves...

06 February 2024 1,525 6 View

What can be the reason of having negative OCR values and warning errors in background wells when running Seahorse?

I'm running XF T Cell persistence assay in a mini Seahorse. I used to not have problems until I start getting some negatives values in OCR measurements, and also errors with both background wells,...

12 January 2024 4,455 0 View

Contact with a teacher or researcher specializing in Sustainability, Fair Trade and Innovation in the trade of agricultural product ?

Good morning, colleague My name is Víctor Hugo Fernández Bedoya, and I am a professor at the National University of San Marcos (Universidad Nacional Mayor de San Marcos), located in Lima, Peru. I...

08 January 2024 1,652 0 View

¿Any suggestion about cryostat slicing issue?

Hello everyone, I need advice on improving the quality of my coronal slices obtained from PFA-fixed mouse brains using a cryostat. As You can see in the attached image, slices are horrible! Any...

04 January 2024 6,071 2 View

Can we preserve the protoplasts?

Can we preserve the isolated protoplasts for future experiments? Is it possible to preserve them via cryopreservation? If yes how long can we preserve them?

16 July 2024 9,576 1 View

What is the composition of cryopreservation medium for virus in -80?

Hello all. I grew ARPE-19 cells in cell culture and infected them with a virus (Varicella-zoster virus). After the virus reached a high infection rate, I harvested everything in the plate and use...

07 May 2024 259 3 View

Why does the SH-SY5Y cell line can undergo an automatisation of differentiating in a regular growth media after being cryopreserved?

I have cryopreserved the undifferentiated condition of SH-SY5Y cell line in the early passage in a cyomedia formulated with 90% FBS and 10% DMSO (sterile-filtered). Some months later, I have...

25 March 2024 1,152 2 View

External or internal threaded cryovial, which one is better for cell cryopreservation?

Hi, I am currently looking for suggestions on choosing which cryovial to use for cell cryopreservation, internal or external threaded, do you guys have a preference? or is there any reason for...

11 March 2024 1,587 1 View

What is the cryopreservation medium approved by FDA?

I know that CryoStore has a food and drug administration (FDA) master file to be use as cryopreservation medium but not sure is there are others? Anyone has an idea?

08 February 2024 3,955 2 View

What could make my CometAssay slides appear cloudy?

I am running the CometAssay on cryopreserved avian blood samples. I had been obtaining what I thought were good results but then encountered a cloudiness problem the first time I tried to do more...

16 November 2023 8,530 0 View

During my cell culture after revival the cells were getting stressed .What might be the reason behind it ?

How long we have to trypsinize A549 Cell as well as Beas2b cell line?? is it due to to fault during cryopreservation ?

28 October 2023 6,894 1 View

Ph.D. direction—choosing between cell cryopreservation, organoids, red blood cells, arteries. Any advice on which area might be suitable for newbie?

"Hello ResearchGate community! I'm a newcomer to the field of research and currently exploring potential research directions for my Ph.D. Among the options of cell cryopreservation, organoids, red...

22 October 2023 5,918 2 View

How long can induced pluripotent stem cells (iPSCs) be cryopreserved for?

This week, a first vial of iPSC derived from hair follicles was thawed, using the same conditions as the last culture method, i.e. coating with Vitronectin and Essential 8 Flex as medium....

31 August 2023 7,027 2 View

Can I cryopreserve using -20C instead of -80C before transferring to -130C (N2 tank)?

Hi, the -80 freezer in my lab is down, it is only reaching -52C at the moment and I need to freeze down some cells. Can I put my vials in the CoolCell and place them at -20C or -50C and then...

24 August 2023 5,210 0 View

Pantu Kumar Roy

Normally researchers used cryovials for cryopreserved with freezing medium. What is the reason to avoid cryovials during preservation?

Macarena Lucia Fernandez Carro

I am creating cell sheet matrixes. So not cells on suspension.

Rihab Abbas Ali

why don't you use the cryovial?

@rihab Ali I am trying to cryipreserve cell sheets. They do no fit in cryovials