6 Questions 9 Answers 0 Followers
Questions related from Sandeep K. Panda
In some cases the -10 of the promoter is known, while in others it is not. In that case how do we predict the transcription start site (TSS)? I have tried using the popular tools available. They...
09 May 2023 3,395 6 View
We know that putting repetitive sequences in a single plasmid may initiate recombination in those plasmids. If recombination happens, the sequences in between those repetitive sequences may get...
18 February 2023 1,045 10 View
I am working with RNA switches for tuning few genes for production of metabolites in Lactococcus. I have observed recombination events in my plasmids when I culture the respective strains for...
09 February 2023 5,583 2 View
I am creating CRISPRi tool for Lactococcus lactis. Do I need to add an extra Lactococcus Terminator sequence at the end of the sgRNA or is the S. pyogenes Terminator enough?
28 April 2021 9,395 3 View
Question pertains to comparison of level of mRNA expression from single gene copy in the genome to single-copy-single-plasmid system. Kindly also explain the reasons for the same.
12 March 2021 3,815 6 View
Multiple ori sequences pertaining to different hosts are usually present in a plasmid. But how many different ones can I have, without causing problems? If problems do get created, what kind of...
26 November 2019 493 2 View