Why Graph Neural Networks aren't hierarchical?

Hello, I read about Graph Neural Networks, and understood that it is very similar to CNN, only that is on graphs and without pooling for downsizing. Each iteration the "convolution" is of...

12 July 2020 4,330 6 View

How can droplet particle type be enabled in ANSYS Fluent?

Hello, I'm trying to enable droplet particle type in Fluent. Energy eqn.s are turned on. However only inert and massless types are available. So, if there is anyone who faced this kind of problem...

22 May 2020 6,387 1 View

Does expired PaxGene tubes have effect on the cfDNA yield or quality?

I have been using a set of PaxGene tubes to collect blood samples from which I have been isolating cfDNA for NGS analysis. I need to collect more blood samples, but I noticed that the expiration...

09 March 2020 3,967 1 View

Which concentration measurement is reliable for cfDNA? Nanodrop or Qubit?

I am trying to isolate cfDNA from plasma samples (2-3 ml) for NGS. I get around 15 ng/ul concentrations of cfDNA from nanodrop but 10 times lower conc (1.4 ng/ul) from qubit dsDNA HS assay. Why...

22 July 2018 7,155 3 View

TRAP staining protocol TROUBLEshooting?

Hi everyone, I am trying to deal with an unreasonable problem with TRAP staining of differentiated RAW264.7 cells (RANKL induced). I was using a protocol for TRAP staining, and it worked perfectly...

12 March 2017 8,140 3 View

PFGE - DNAse contamination?

26 October 2016 9,269 3 View

Converging problem in transient heat analysis due to amplitude. Anybody has idea?

15 January 2016 3,765 2 View

What is the typical concentration of total proteins isolated from cell lines, say L929?

12 September 2015 476 2 View

Is there any publicly available benchmark dataset to check software code plagiarism?

A plagiarized code may be either an exact copy of the original code, or a variant, applying various textual transformations such as; - Changing the order of operands/operators in expressions,-...

02 February 2015 9,851 7 View

Can anyone tell me the ingredients of liver cancer stem cell media?

26 October 2014 9,662 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How can we limit artifacts caused by rotation in fiber photometry?

We are trying to run fiber photometry experiments for long time scales, but we get these sinusoidal artifacts that come up. We think they may be caused by twisting of the fiber optic subject...

25 February 2021 568 1 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View