CRISPR for large deletions in bacteria?

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Tomasz Cłapa

Hi Deepak,

We did not do it yet, but for sure we are going to try it next year. I have a project about P. aeruginosa and it's resistance and we are going to try delate some parts of it's genome. So, please be in touch if your are interested, and we will see how it is developping.

cheers,

Tomasz

Samy Selim

Can I follow your question

yes, sure

Deepak Balasubramanian

@Tomasz Cłapa Great! For sure, I'll keep in touch.

I found an article where they've done it using nickases instead of the typical dsDNA breakage (DOI: 10.1021/acssynbio.5b00132). Is your planned approach similar?