Deepak Balasubramanian

2 Questions 6 Answers 0 Followers

Questions related from Deepak Balasubramanian

CRISPR for large deletions in bacteria?

Hi, I'm wondering if anybody here has used CRISPR in bacteria (Vibrio/Pseudomonas) to delete large (>10 kb) chunks of DNA. I appreciate any pointers/resources/commercial kits. Thanks!

31 October 2018 9,360 4 View

Can anyone help with long primer PCR with ~25% target complementarity?

I am having trouble with a couple of PCRs where the primers are long (Fwd 45 bp, Rev 90 bp); 25% of the primers are target complementary. The Tms for the complementary regions are 64˚C and 66˚C...

03 April 2014 3,825 7 View