I need to insert a new set of sgRNA sequences in  CRISPR/Cas9 Lentivector,.

I'll do this by designing primers to replace the existing 20bp sgRNA sequence with the one of interest.

I'll design the primers with http://nebasechanger.neb.com/ based on the vector sequences, CRISPR sequences that I have already.

So far so good...

My questions:

Once I've amplified successfully (which I plan to do using a Phusion enzyme master mix!?! is this ok?) some protocols suggest I need to ligate with T4, some suggest I need to digest with DpnI, some suggest I need to do both.

Do i need to ligate?

Do I need to digest?

Do I need to both?

...before I transform my bacteria.

Thanks