Crispr Cas 9 service

More Joost Boeckmans's questions See All

Bonferroni: pairwise comparisons within multiple subsets of data, what do I divide 0.05 with?

I have a nominal variable that divides my data in 8 groups, 1 to 8. Another nominal variable divides my data in 2 groups, A and B. Each combination of these two exists, 1A , 1B, 2A, 2B, etc. The...

14 March 2022 6,112 0 View

How to perform a power analysis for a clinial trial using ANCOVA?

Upon reading following paper: https://pubmed.ncbi.nlm.nih.gov/31727409/, I saw that the power analysis is based on a two-sample t-test, while the primary endpoint is based on ANCOVA analysis...

28 September 2021 8,660 6 View

Looking for bilateral FDI data between China and (Sub-Saharan) African countries?

Dear reader, as the title says I am looking for FDI data between China and (Sub-Saharan) African countries. To be more precise: I need FDI data from China to various seperate (Sub-Saharan) African...

22 September 2021 8,807 7 View

T cell inflamed gene expression profile: how to calculate?

Dear colleagues, I want to calculate the T-cell inflamed Gene Expression Profile (GEP) score as described by Ayers M, Lunceford J, Nebozhyn M, et al: IFN-g-related mRNA profile predicts clinical...

05 September 2021 5,305 0 View

What are these structures within the roots of Cerastium semidecandrum, mycorrhizal structures or something else?

I have trouble figuring out what these structures are within the roots of Cerastium semidecandrum. I am working on a project that aims to determine the differences in (arbuscular) mycorrhizal...

20 May 2021 3,606 2 View

Is there such a thing as Igg specific MHC class II epitopes or do all non-self MHC class II epitopes trigger antibody production of all subtypes?

I know there are bioinformatic tools that allow epitope prediction to determine if a certain peptide would trigger an immune response. I also read that for allergen diagnoses IgE of sensitised...

09 February 2021 4,398 3 View

What are the most efficient loxP sites for cre-mediated inversion in mice?

Hi, We want to make a mouse which has loxP flanked construct. Upon Cre expression this construct need to inverse. I'm wondering which loxP sites are best to flank the construct with... Does...

24 February 2020 2,591 0 View

Silver Thiosulphate synthesis, how to?

I need to prepare a solution with Silver Thiosulphate. So far it fails, and the solution has black particles in it. Can anyone provide me with some practical guidelines to make this work? I tried...

21 March 2019 3,226 1 View

In a lineair Mixed model (spss) I would like to add in interaction effects, however due to a large number of combination, many become 'redundant' ?

Any alternatives / options to solve this? Because of individual interaction examinations i know that these interactions exist, but combining all interactions gets me in trouble. I have both...

13 April 2016 4,555 3 View

What method is used to calculate the reflectance factors of the calibration panel?

In the publication the Rpλ withequation 7 from the remote sensing of land surfaces course BIE 6250. The acquisition of these individual reflectance factors is not explained. Could anyone explain...

08 December 2014 5,715 4 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 639 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,892 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

Luca Mologni

Dear Joost,

for CRISPR products and services I usually turn to Horizon Discovery or GenScript.

See this page for custom gene-edited cell lines development:

https://www.horizondiscovery.com/cell-lines/cell-line-engineering

I hope this is useful

best,

Luca

Joost Boeckmans

Luca Mologni Thank you very much!