T cell inflamed gene expression profile: how to calculate?

06 September 2021 0 5K Report

Dear colleagues,

I want to calculate the T-cell inflamed Gene Expression Profile (GEP) score as described by Ayers M, Lunceford J, Nebozhyn M, et al: IFN-g-related mRNA profile predicts clinical response to PD-1 blockade. J Clin Invest 127:2930-2940, 2017.

In their article, they describe that this score is calculated as a weighted sum of

normalized expression values for the 18 genes. Does anyone know how you perform such a weighted sum? (The 18 genes are: PSMB10, HLA-DQA1, HLA-DRB1, CMKLR1, HLA-E, NKG7, CD8A, CCL5, CXCL9, CD27, CXCR6, IDO1, STAT1, TIGIT, LAG3, CD274, PDCD1LG3, CD276).

Thanks in advance!

Joost Koedijk

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