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Questions related from Pedro Cunha
I will isolate mouse naive T cells and, prior to activation, I would like to KO a doubled floxed gene. Has anyone ever tried this, in T cells or in another suspension primary culture...
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Helo dear colleagues After transfection and a 48h drug treament we want to assess the motility of our Glioblastoma cell lines. Is it important to use a ECM coating to perform this assay? (during...
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What we pretend are primers for qRT-PCR. The secondary structure of ncRNAs makes it difficult to design primers with regular programs such as Beacon ins't it?
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We add resazurine to the following conditions: Just media (neg Control) Non-transfected cells (our positive control) Non-transfected cells + drug Transfected cells Transfected cells + drug To...
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In my lab we are interested in fighting glioblastoma pretty hard. Now we are using two pan-tyrosine kinase inhibitors, namely Sunitinib and Axitinib. Because one of the major in vivo tumor...
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I'm currently working with isolated Glioblastoma stem-like cells and in order to make sure they are in fact what I think they are, I would like to see if they indeed divide assymetrically. I now...
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