I want to soluble completely silk fibroin. Suggest me solvent that will completely solubilize silk.
Hai
We dissolved it using a ternary solvent after degumming , Calcium chloride:ethanol:water in 1:2:8 molar ratio, at a temperature of 55 degree Celsius for 4 hours with continuous stirring.
All the best
Mamatha
We use 9.3M LiBr to dissolve our silk fibroin to make a final concentration of 25% SF in the 9.3M LiBr. We do this at 60C for 4 hours, as according to David Kaplan's protocol. To dissolve tougher silk fibroins, you can try higher temperatures or higher concentrations of the LiBr.
Organic solvent: Hexafluoro Iso propanol is very good for dissolving silk fibers!
Other salt based procedures are time consuming! (bcoz od dialysis)
Formic acid is also a better solvent!
May I ask a question? How is the reaction of silk fibroin and lithium bromide to dissolved fibroin?
I have tried with Calcium chloride:ethanol:water in 1:2:8 molar ratio, at a temperature of 55 degree Celsius for 4 hours with continuous stirring. Silk fibroin formed aquous solution by using ternary solvent system.
What concentration, temperature, time should i give if i were to dissolve two cocoon silks?
1) Hi you can try mixture of trifluro-acetic acid and chloroform.
2) Eri silk was degummed by immersing in the sodium carbonate solution and kept in 25 degree C and at a pH level of 8.5-9.0, for 30 min to remove the sericin content.
The solvent which i used (9.3M LiBr) for dissolving degummed Antherea mylitta (Tasar) silk fibroin did not completely dissolved in said solvent.
Whether the fibres will dissolve completely or partially?
If partially do i need to cetrifuge or filter the sample?
I successfully dissolved 2.5wt% silk fibroin in 28% ammonium aqueous solution by magnetic stirring.
Hi..
How to prepare calcium chloride:ethanol:water in 1:2:8 molar ratio?
Please i am having the same problem. Give the detail and type of ionic liquid to be use in dissolving spider silk
If we will use LiBr, then how to remove it from final solution?
Thanks Mr. John George Hardy for providing ample literatures.
Kindly suggest me how to remove formic acid solvent from dissolved demmugged silk. Are there any methods other than dialysis?
does these procedures apply to any other protein system having beta structure?
how can I solubilize a protein with beta sheet structure so that it will degrade faster in environmental conditions?
You could use LiBr 9.3M or a mix of CaCl2/Water/Ethanol. It is also soluble in HFIP.
thank you Catalin....will this decrease its strength and other mechanical properties?
can we use lithium chloride for this purpose instead of lithium bromide?
We used the trinary solvent (CaCl2, H2O,C2H5OH ) during 3 hours and heated to 60 c ...silk cocoons didn't solve completly....how much does it take to dissolve them compeletly!the changes after 3 hours are so negligible....!what should I do?
use liBr 9.5 molar solution for 6 hrs and 80 C temperature. then for removal of liBr from that solution use ethanol. fibroin will settle at the bottom and decented out.
CaCl2:EtOH:H2O (1:2:8 molar ratio) for degummed fibers added in concentrations up to 10 %w/v would take approximately 4-5 hours at 60-70 deg C. If it did not dissolve well, there could be some unwanted sericin. Attempts of going above the 10% w/v mark might not dissolve completely (the limitation to using this protein). Also ensure the solvents are prepared fresh.
9.3 M LiBr works efficiently and has shown to be more stable in this solvent compared to CaCl2:EtOH:H2O. Like the solvent above this can dissolve up to 10% w/v deggummed silk. For purification, following dialysis vs ultrapure water, this concentration goes down to 4-5% w/v over a span of 2-3 days.
EDIT: I've also used LiCl, and found that this would work efficiently in DMF (4.0 M concentration).
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