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Questions related from Maria Christine Tankeh Asuncion
I'm trying to do a gene expression study for porcine mesenchymal stem cells which have been induced towards differentiation using different chemical agents. At first, I tried to design my own...
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I'm using porcine bone marrow-derived mesenchymal stem cells and when I have excess cells, I freeze them down. When I thaw them again for the purpose of conducting experiments like...
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I want to do immunocytochemistry with mesenchymal stem cells. I've been reading through different protocols and some mention having to coat their coverslips with gelatin or fibronectin whereas...
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I'm trying to follow the methodology in one paper that describes the staining of cardiomyocytes with anti-connexin 43. After the procedure for staining, the paper states that "Samples we're...
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We tried to compare the absorbance values of several copper sulfate standard solutions using Evolution 220 spectrophotometer (the traditional one), and the Nanodrop, but we we're surprised to find...
04 April 2014 1,653 4 View
I would like to compare the change in the absorption spectrum of a protein with the change in pH. However the protein concentration in one pH is not necessarily equal to the concentration at a...
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The literature I'm reading stated that they dialyzed their protein solution in a borate buffer (100mM borate, 150 mM NaCl, pH 9). They didn't state the volumes used, so I read up from other...
12 December 2013 628 18 View
I would like to synthesize propargyl methacrylate for research involving click chemistry. I wanted to buy it, unfortunately it's classified as a "Dangerous Product" in Singapore and the chemical...
11 November 2013 1,150 15 View
How do you prepare a borate buffer (100 mM borate, 150 mM NaCl, pH 9)? How would I know what system to use (e.g. boric acid-NaOH, sodium tetraborate-HCl, boric acid-sodium tetraborate, etc.) if it...
11 November 2013 4,921 11 View
I'm planning to add functionalities to a protein molecule using certain amino acids in its backbone. I would just like to ask, why is it stated that "it is essential that amino acid building...
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