Could you please suggest a simple and rapid method for plasmid isolation from yeast?

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Evelyn Prugar

If you have the money buy the MasterPure Yeast DNA purification kit from epicentre an illumina company (www.epicentre.com) Cat number MPY80200 (USA). The DNA quality is high and easy to do.

Cheers, Evelyn

Behrooz Moosavi

Dear Evelyn

Thank you very much for the reply. As my funding is limited I am looking for a cheap and fast method. I came across several methods published in papers. One of them was based on glass beads but the rersult was not very promising. I wonder if you or others have any good experience with cheap methods. Thank you very much.

Hi Behrooz,

I have attached my protocol that I have had great success with. It does involve glass beads. Let me know if you have any questions.

Cheers,

Evelyn

Many thanks Evelyn. I will try and see how it goes.