Is there any way to quantify filamentation in Saccharomyces cerevisiae? For example a mutant might be a better filament generator than the other one?

More Behrooz Moosavi's questions See All

I wonder if you could send a protocol for recovering protein from a yeast culture supernatant meant to include a secreted protein?

The aim is to purify protein by his- tag purification. I assume it requires a protein concentrating step before laoding on affinity column.

11 December 2017 3,674 8 View

I wonder what protein purification method is best for purifying a heterlogous protin about 100 kd in yeast?

The effect of the size of a protein on choosing a purification method

10 November 2017 6,770 9 View

I wonder if there is a simple non-radioactive assay for studying global protein synthesis in yeast?

I am looking into any possible defect in protein synthesis caused by a drug in yeast.

31 December 2015 7,512 5 View

Could you please suggest a simple and rapid method for plasmid isolation from yeast?

I need to recover the plasmid to check for any potential changes in the sequence.

03 April 2014 1,892 4 View

Yeast growth on selective media

We transformed a yeast with a plasmid harboring the ura marker (pYES2 and pYES2+Hsp104). The transformed yeast appears on -ura plate after 5-6 days. However when colonies are used to inoculate the...

02 March 2013 7,694 21 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

London dispersion in CCl4 vs the dipole-dipoles force strength in CHCl3?

How do you tie-break and rank these two in their strength?

03 August 2024 4,471 1 View

What are the limitations and challenges of using machine learning for predicting concrete compressive strength in practical applications?

Machine learning (ML) has shown great potential in predicting the compressive strength of concrete, an important property for structural engineering. However, its practical application comes with...

03 August 2024 2,546 2 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Hello researchers, how can one get the optical density of a filamentous algae?

i am comparing the growth performances of filamentous and non filamentous forms. in a case where I can not count the cells of the filamentous algae using a hemocytometer, is it okay to do a...

30 July 2024 5,985 2 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

A twin-shaft kiln or a rotary kiln for lime production 1 class? if compressive strength of limestone is low 60 MPa?

My research work is related to the calcination of limestone. I have a complex question and would like to consult with experts in this field. Could you please advise which kiln to choose (a...

18 July 2024 1,390 2 View

Han Zhang

Hi Behrooz, you can measure actin filaments (F-actin) using FACS. Here I'd like to refer you to one of my publication where indicates the methods in detail:

Article Hedgehog inhibitors selectively target cell migration and ad...

Hope it would help you!

Behrooz Moosavi

Thank you very much Han. Is there any simpler way than this method, applicable to high throughput experiments? (as there is no FACS around.)