Is there any way to quantify filamentation in Saccharomyces cerevisiae? For example a mutant might be a better filament generator than the other one?

More Behrooz Moosavi's questions See All

I wonder if you could send a protocol for recovering protein from a yeast culture supernatant meant to include a secreted protein?

The aim is to purify protein by his- tag purification. I assume it requires a protein concentrating step before laoding on affinity column.

11 December 2017 3,615 8 View

I wonder what protein purification method is best for purifying a heterlogous protin about 100 kd in yeast?

The effect of the size of a protein on choosing a purification method

10 November 2017 6,702 9 View

I wonder if there is a simple non-radioactive assay for studying global protein synthesis in yeast?

I am looking into any possible defect in protein synthesis caused by a drug in yeast.

31 December 2015 7,460 5 View

Could you please suggest a simple and rapid method for plasmid isolation from yeast?

I need to recover the plasmid to check for any potential changes in the sequence.

03 April 2014 1,838 4 View

Yeast growth on selective media

We transformed a yeast with a plasmid harboring the ura marker (pYES2 and pYES2+Hsp104). The transformed yeast appears on -ura plate after 5-6 days. However when colonies are used to inoculate the...

02 March 2013 7,622 21 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Concrete compressive strength databases?

I am looking for datasets similar to this one: http://archive.ics.uci.edu/ml/datasets/Concrete+Compressive+Strength I am looking for concretes with different types of aggregates (Recycled,...

27 February 2021 6,801 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

What are effects of steel jacketing on flexural strength?

Dear all, If steel jacketing is used to retrofit a reinforced-concrete (RC) column, can we use it to improve flexural strength? I understand that we leave gaps between the ends of the jacket and...

22 February 2021 1,906 5 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Han Zhang

Hi Behrooz, you can measure actin filaments (F-actin) using FACS. Here I'd like to refer you to one of my publication where indicates the methods in detail:

Article Hedgehog inhibitors selectively target cell migration and ad...

Hope it would help you!

Behrooz Moosavi

Thank you very much Han. Is there any simpler way than this method, applicable to high throughput experiments? (as there is no FACS around.)