Behrooz Moosavi

6 Questions 19 Answers 0 Followers

Questions related from Behrooz Moosavi

Is there any way to quantify filamentation in Saccharomyces cerevisiae? For example a mutant might be a better filament generator than the other one?

I'd like to compare filamentation strength in yeast and need an assay to compare different strains.

01 January 2018 7,439 2 View

I wonder if you could send a protocol for recovering protein from a yeast culture supernatant meant to include a secreted protein?

The aim is to purify protein by his- tag purification. I assume it requires a protein concentrating step before laoding on affinity column.

12 December 2017 3,656 8 View

I wonder what protein purification method is best for purifying a heterlogous protin about 100 kd in yeast?

The effect of the size of a protein on choosing a purification method

11 November 2017 6,757 9 View

I wonder if there is a simple non-radioactive assay for studying global protein synthesis in yeast?

I am looking into any possible defect in protein synthesis caused by a drug in yeast.

01 January 2016 7,499 5 View

Could you please suggest a simple and rapid method for plasmid isolation from yeast?

I need to recover the plasmid to check for any potential changes in the sequence.

04 April 2014 1,877 4 View

Yeast growth on selective media

We transformed a yeast with a plasmid harboring the ura marker (pYES2 and pYES2+Hsp104). The transformed yeast appears on -ura plate after 5-6 days. However when colonies are used to inoculate the...

03 March 2013 7,656 21 View