I am working on a grant application and looking for a practical recommendation regarding oxygen sensors for cell culture media and tissue (tissue engineering constructs). I don't specifically need continuous monitoring, just measurements at certain conditions/time points. Thank you!
Hello Anna Guller
You could refer to the links below. It may be helpful.
The Measurement Principle
The measurement principle can be explained as follows.
At the core of this technique is the indicator: a fluorescent analyte-sensitive dye. That dye is embedded in a polymer matrix so that it does not leach into a culture medium or sample. The indicator is excited with light of a certain wavelength and emits fluorescence in response. According to the amount of analyte molecules present, dye changes its fluorescence signal. That change in signal is measured by a respective electro-optical reading device and can be translated into concentration values. Optical fibers are used to transfer excitation light to the indicator dye and sensor response back to the reading device. The indicator inside its polymer matrix can be coated on a carrier material or directly onto the distal end of an optical fiber so that different sensor designs can be achieved.
https://bioprocessintl.com/upstream-processing/bioreactors/using-optical-sensors-for-bioprocess-monitoring-a-measurement-technique-for-bioprocessors/
https://www.labcompare.com/812-Dissolved-Oxygen-Analyzer-Dissolved-Oxygen-Monitor/3056-SDR-SensorDish-Reader/
I hope it helps.
Best,
Firesting O2 meters by PyroScience are very good, you can easily set the sampling rate with the included software.
https://www.pyroscience.com/en/products/all-meters/fso2
oxygen sensors for cell culture media and tissue : Thin film oxygen sensor
The gold standard here has been the OxyLite for some time as the system is highly published and widely used in many fields including tissue engineering. Unlike some other systems the sensors do not consume any O2 and do not need to be calibrated. https://www.oxford-optronix.com/assets/files/References-November-2021.pdf
There is bound to be a few papers there that you can use as reference.
https://www.oxford-optronix.com/oxylite-oxygen-monitors
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